Maria Lung (University of Hong Kong, Hong Kong SAR China)
“New Insights in NPC Detection Utilizing Circulating Tumor Cell Enumeration and NGS Analysis”
NPC AoE Research Tissue Bank (http://www.cnpcr.hku.hk/tb1.htm)
- CTCs and CtDNA (Dr. Lin, JC and Dennsi Lo) provide different and complementary information
- Cancer Research 2013. (Pastel and Alex Panabieres) Real time liquid biopsy in cancer patients: fact or fiction
- CTC Number. Cell type
- 1-1000. CTCs
- 50 million. WBCs
- Like finding you among 7.4 million people
- Enrichment of CTCs by microfluidic device by size
- —> CK and EpCAM + CD45 (excluding lymphocytes)
- NGS-based SeqCp targeted capture approach (nimble gene)
- Summary of SeqCap NGS pipeline (Dai Wei)
- Chromatin regulator, Ubi conjugation, tumor suppressor
- Amplification -based HeatSeq oncology panel – 60 genes for validation and discovery for CTC samples with low purity
- Clinical implications of bold-based biopsy monitoring in metastatic NPC patients
- Purpose:
- 1. Investigate the prognostic and predictive value of TC counts and CTC NGS analysis at diagnosis during and at eh end of CT
- 2. Determine whether liquid biopsy serial monitoring allows more sensitive and early detection of relapse than imaging
- 3. Characterize the genetic changes and signature of
- Four serial time-point schedule for CTC analysis of NPC patients with disseminated disease treated with palliative intent
- [Study Overview]
- May 2016 – May 2018: recruited 20 disseminated NPC patients prospectively from 3 hospitalsmutations. Out of 72 NOC-relevant genes from previous 3 NGS studies (Lin DT Singapore, KW Lo and Maria Lung)
- KMT2C, FAT1, EP300
- Mutations with progressive diseases (PD)
- Chromatin regulator
- Cell proliferation
- TGFb signaling pathway
- MAPK
- Genes associated with complete response (CR)
- DNA damage
- Immune