由 sufang 在 六, 11/22/2014 – 09:01 發表 Cholangiocarcinoma cholangiocarcinoma FFPE Gene Fusion
Nov 5 2014
Daw-yang Hwang 黃道揚 to LTCHEN, yngmiin
Dear Prof Lin, Dr. Chiang, and SF:
I designed primers for ALK, ROS-1 FGFR2, FGFR3, and I can add primers for RET.
In my research part (bladder and upper urinary tract cancer), I am collaborating with urologists in KMUH. Hopefully I can get cDNA samples from then late this month. I can run the above panel for cholangiocarcinoma, oral cancer, or any cancer samples you are interested. I am currently testing some FFPE samples.
Brief, the protocol:
1. Samples (fresh or FFPE)
2. Extract RNA
3. RT-PCR for single-starnded cDNA
4. second cDNA systhesis (double-stranded cDNA)
5. Nextera XT kit
6. Specific PCR amplification
7. Barcode PCR (up to 288 different samples in one MiSeq run)
8. Purification
8. MiSeq run
In my research part (bladder and upper urinary tract cancer), I am collaborating with urologists in KMUH. Hopefully I can get cDNA samples from then late this month. I can run the above panel for cholangiocarcinoma, oral cancer, or any cancer samples you are interested. I am currently testing some FFPE samples.
Brief, the protocol:
1. Samples (fresh or FFPE)
2. Extract RNA
3. RT-PCR for single-starnded cDNA
4. second cDNA systhesis (double-stranded cDNA)
5. Nextera XT kit
6. Specific PCR amplification
7. Barcode PCR (up to 288 different samples in one MiSeq run)
8. Purification
8. MiSeq run
Maybe we can meet sometimes to have a discussion, for:
A) where to run MiSeq
B) discuss whether we want to include potential fusion on both ends or run known fusion-direction.
A) where to run MiSeq
B) discuss whether we want to include potential fusion on both ends or run known fusion-direction.
Kindly regards,
Daw-yang
Daw-yang
Nov 5 2014
Su-Fang Lin to 陳所長
Su-Fang Lin
Dear 所長:
那我就向黃秀芬醫師申請TLCN的cholangioncarc inoma sample cohort做:
1) FGFR2 IHC 染色 (我們剛剛買了一些commercial的tissue array, 在測試抗體條件)
2) cDNA for 道揚’s RT-PCR test.
相請教您的是,用您的名字申請? 還是用我的?
Best,
Su-Fang
Nov 6 2014
Dear SF,
Nov 6 2014
Su-Fang Lin to Daw-yang Hwang
Daw-yang Hwang 黃道揚 to me
I will check my user name in the NHRI system.
Nov 6 2014
Su-Fang Lin to Daw-yang Hwang
Nov 6 2014
Daw-yang Hwang 黃道揚 to me
Su-Fang Lin to Daw-yang Hwang.
Thanks a lot for the valuable info! And sorry I had been in a meeting whole afternoon, in case you had called me.
Best,
Su-Fang
Su-Fang
11/05/2014