Fig. 1. CTCF repressed the lytic cycle replications of EBV and KSHV in viral latently infected cells.
> (a). The band of CTCF ectopic-expressed cells is over-exposed.
> (b). The expression level of shCTCF is similar to shLuc.
Fig. 2. Rta binding sites are similar to CTCF binding sites
Fig. 3. 4. EBV Rta expression increased DNA methylation on the target cellular promoter thus interfered with the occupancies of CTCF.
> (a). RNA expression kinetics of cellular genes should be showed in parallel.
> (b). To confirm the methylation states of cellular promoters in EREV8 cells, especially at 24 h of Dox induction.
Fig. 5. Rta expression induced CpG methylation on LCR, RCR, and oriLyt region in both EBV and KSHV genomes that influenced CTCF bindings.
> (a). RNA expression kinetics of viral genes around LCR/RCR/oriLyt region should be showed in parallel.
> (b). To move the model figure to the last one.
> (c). To confirm the CTCF binding on EBV RCR.
> (d). To confirm the DNA methylation states of EBV LCR, KSHV RCR
Fig. 6. EBV Rta associated with DNA methyltransferase, DNMT3A and DNMT3B.
> In Fig. 6B and 6C, the expression level of viral immediate-early proteins were not equal. In addition, normal rabbit IgG should not interact with any viral proteins. It is necessary to improve the wash step through IP experiment.