Henri-Jacques Delecluse (DKFZ – German Cancer Research Center, Germany) “Is NPC Caused by a Particular EBV?”
- Kowk et al 2014 J Virol phylogenetic tree
- Palsler et al JV 2015 EBV polymorphism
- M81 and B95-8 difference Tsai et al Cell Reports 2013 ((Tsai et al., Cell Rep, 2013 Spontaneous lytic replication and epitheliotropism define an Epstein-Barr virus strain found in carcinomas)
- J Mautner will be reporting in Switzerland regarding epitope polymorphism M81 B958 AG (B95-8 and M81: CD4 epitope only 30% conserved; CD8 only 70% conserved.)
- The mucosa that covers sinuses is a good source of respiratory
- Transfer infection vs direct infection
- Involcurin is a marker for late differentiation
- M81 and B95-8 express gp110 at different levels
- The Epstein-Barr Virus BART miRNA Cluster of the M81 Strain Modulates Multiple Functions in Primary B Cells ((Lin et al., PLoS Pathog, 2015 The Epstein-Barr Virus BART miRNA Cluster of the M81 Strain Modulates Multiple Functions in Primary B Cells)
- M81 replicates in vivo
- Functions served by the BART miRNAs
- BART miRNAs inhibit BZLF1 expression
- The BART miRNA also inhibits LMP1 expression
- Deletion of the BART miRNAs increases infectivity in NSG mice
- BART inhibit LMP1 expression
- Pro-apoptotic (caspase 3) vs anti-apoptotic (LMP1)
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- Polymorphism issue
- 14 EBVL, 9 IM, GP202 Korean gastric cancer, 30 days – 40 days all NSG mice died. Except some.
- Replicating viruses are more tumorigenic in vivo
- Aneuploidy after M81 infection: M81 WT > delta ZR
- Cliona Rooney commented: John Sixby published BCLF1 increases aneuploidy
From Ming-Han
From HJD news:
B95-8 and M81: CD4 epitope only 30% conserved; CD8 only 70% conserved.
Sphenoidal sinus (Respiration sinus)
Question: BART and epi cells? ongoing
Q: try to study polyorphisms? Yes
Q: by Paul Lieberman why M81 more lytic but in Asia lymphoma is low?
A: the balance between grow rate and lytic replication?
In EBV Meeting
Session 2: B Cell Infection
The Epstein-Barr virus BART miRNA cluster of the nasopharyngeal carcinoma- associated M81 strain modulates lytic replication and cell growth in primary B cells
Ming-Han Tsai, German Cancer Research Institute, Heidelberg, Germany
Session 13: Adaptive Immunity to EBV
Epstein-Barr virus strain heterogeneity impairs T cell immunity
Josef Mautner, Helmholtz Center Munich, Germany