Report (2003) by Prosperi’s group
Title is: p21 does not interfere with loading of PCNA at DNA replication sites, but inhibits subsequence binding of DNA polymerase delta at the G1/S phase Transition.
By tagging a EGFP fragment onto the N-terminus of p21, the authors showed that
location of EGFP-p21 is in the nucleus of HeLa, and most cells were arrested in G1 or G2/M compartments. The inhibition of DNA synthesis was demonstrated by BrdU labeling. i.e. pulse labeled BrdU followed by anti-BrdU antibody (drmatically reduced). Further, CCNA was found to be downregulated by p21.