SFL

寄送SW780 RNA給長庚及行動基因陳華鍵

寄送SW780 RNA給長庚及行動基因陳華鍵由 sufang 在 五, 02/13/2015 – 15:20 發表 Cholangiocarcinoma GeneFusion From: Su­Fang Lin [mailto:sflin1@gmail.com] Sent: Friday, January 30, 2015 2:06 PMTo: angel945@cgmh.org.twSubject: this is Su­Fang’s email Dear Dr. 趙:This is my email. I will prepare ~5­10 micrograms RNAs from SW780 cells for your control. Please let me know to whom and to where should I sent. Thanks! Best, Su­Fang  ————————————– Angel […]

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Fusion gene detection 2014-Dec 及 TLCN檢體申請

Fusion gene detection 2014-Dec 及 TLCN檢體申請由 sufang 在 三, 01/21/2015 – 11:29 發表 Cholangiocarcinoma cholangiocarcinoma FGFR2 gene fusion Nov 27, 2014 at 2:57 PM To: Su-Fang Lin Dear SF, Only minor suggestions (in red color). Are you going to list the oncogenes screening in your proposal or we can just perform the exam? I am

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Collaborative Projects between KMUH and NICR

General Fluidigm Access Array + MySeq –YouTube 1: https://www.youtube.com/watch?v=s9HUhuCbbhU –YouTube 2: https://www.youtube.com/watch?v=WXpNjcRwdj8 –AA_Illumina_ug_100-3770 (pdf) –RecoverAll Total Nucleic Acid Isolation Kit for FFPE (pdf)   台大郭頌鑫醫師抽取DNA and RNA from tiny endoscopic biopsy samples使用。 –陳華鍵老師他們新開的公司行動基因,用的是類似方法、只是不同機器(Ion Torrent PGM/Proton) –Archer: Archer™ FusionPlex™ FGFR Panel (USD 495 / 8 rxn) –Life: Ion AmpliSeq™ RNA Fusion Lung Cancer Research Panel –與台大合作部分,詳見HPV and p16 in HNSCC. (按我連結)  –ds-cDNA protocal from Yi-Mi and Dan (03/05/2015). (按我下載)

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癌症個人化醫療前瞻論壇 @ 台北喜來登 星月廳 (B2F)

20150111_癌症個人化醫療前瞻論壇 @ 台北喜來登 星月廳 (B2F)由 sufang 在 日, 01/11/2015 – 20:41 發表 Pre-published Meeting Oral Cancer Opening remarks: 賴瓊慧教授 (副研發長) 主持人: 王子豪醫師 (國外廠商contact him about STIP1) 閻紫宸教授: 台灣口腔癌病患新穎癒後生物標誌的發現與臨床意義 –Why OSCC: Taiwan has the oral cancer incidence in men worldwide –Oral cavity cancer: incidence, survival and treatment –廖俊達教授is the head of CGH HNSCC group leader (pastic

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與IBPR開會小記

由 sufang 在 五, 12/05/2014 – 10:12 發表 Pre-published IBPR Meeting 杏國Syncore (DBPR204, DBPR 104) 健亞Genovat (DBPR108, T2DM) 中天(Microbio)   cKIT, IDO1, IDH1, 大分子   COE of New Drug Discovery Success in clinical trials and regulation approval Drug discovery engine Team of excellent investigators Mentor/coaching/networking Triangular relationship (Target, Drug, Disease) PK/PD correlation Biomarkers Translational model

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Fusion gene detection

由 sufang 在 六, 11/22/2014 – 09:01 發表 Cholangiocarcinoma cholangiocarcinoma FFPE Gene Fusion Nov 5 2014  Daw-yang Hwang 黃道揚 to LTCHEN, yngmiin  Dear Prof Lin, Dr. Chiang, and SF: I designed primers for ALK, ROS-1 FGFR2, FGFR3, and I can add primers for RET.In my research part (bladder and upper urinary tract cancer), I am collaborating

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CTCF and HOXs

由 sufang 在 四, 11/20/2014 – 12:53 發表 EBV and KSHV CTCF Mol Cell Biol. 2014 Oct;34(20):3867-79. doi: 10.1128/MCB.00567-14. Epub 2014 Aug 18.CTCF controls HOXA cluster silencing and mediates PRC2-repressive higher-order chromatin structure in NT2/D1 cells.Xu M1, Zhao GN1, Lv X1, Liu G1, Wang LY2, Hao DL1, Wang J3, Liu DP4, Liang CC1.Author informationAbstract HOX

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Remarkable Role of Indoleamine 2,3-Dioxygenase and Tryptophan Metabolites

由 sufang 在 四, 11/20/2014 – 13:20 發表 Pre-published IDO1 KYN Remarkable Role of Indoleamine 2,3-Dioxygenase and Tryptophan Metabolites in Infectious Diseases: Potential Role in Macrophage-Mediated Inflammatory Diseases (PubMed) (pdf 4235) Figure 1. Schematic overview of the kynurenine pathway. It is estimated that only 1% of dietary tryptophan (TRP) can be converted into serotonin (5-HT). The

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Protocols-including KDF

#1由 sufang 在 一, 05/18/2015 – 08:50 發表。 Keratinocyte culture method   說在前面: Rheinwald Lab所寄給我們的Human Keratinocyte Culture Methods第一段便比較下面三個廠商來的media, 說請大家用K-sfm來養”low-to-moderate density”的keratinocytes (primary or immortalized) K-sfm Life Technologies grow well from low to moderate density EpiLife Life Technologies   KGM (or MCDB154) Clonetics very short replicative lifespan    第二段: 須牢記於心的是, K-sfm的營養成分僅適用於低密度、聚集性的keratinocyte生長, 因此用K-sfm養的細胞, 1/3滿度前就必須passage.   第三段: 碰到OSCC的話, 建議與irradiated 3T3

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