SFL

我的題目如下，然後 3/12 departmental talk 直接跳過不用講~ (德政!) Epstein-Barr Virus Rta-Mediated Oncogenic Herpesvirus Reactivation in Epithelial Cells殘念的是，DDR1 和 oral cancer 部份無法跟大家分享了，下次再說~

2011/10/6–7 Rehearsal2011/10/29–30 Scientific Review/Site Visit2012/2/17–18 PI Retreat Review Member:鄭院士、龔院士、楊院士、劉院士、閻校長、洪院士、院長、王副院長 PI 23 RA/postdoc 20 Director Chang JYDrug discoverypast 3-yr publicationHuR bind to HIF1a mRNA stablization (inhibited by MPT0B098)MPT0B098 decreased cytoplams translocation of HuRMPT0B292 HDAC6 inhibitorHDAC6 acetylates a-tubulinCa2+ influx STIMI Ac actinWhat is the effect of actin acetylation upon STIM1 activation?nticancer efficacy of PLGA-30MGMT modulates therapeutic efficacy

劉醫師和周博士pass給我的paper. Ref 1: EBNA 1 MoAb 2B4 cross-reacts with MAGE4. Ref2: Metformin inhibits NPC cell C666-1 proliferation.

小嬿: (A) 搞定EBV Rta 對 CTCF 在 genome organization 之影響。 (B) 行有餘力 (and a hobby!): Establishment of TW05-EREV Jean: (A) Find out the best differentiation protocol for NP460hTert. (1) reagents: TGFb, calcium … (2) markers: p63, MYC… (3) controls: TW05TetER and TW01TetER #16 cells (4) Ref: 第一篇，第二篇 , 第三篇… (B) 行有餘力: Cloning of TW05-EREV (由小嬿、小夏指導)小夏:Continue

Dan介紹的ChIP-Seq皇后網站。 參考一下她的Protocol專區。

(AC503, Ingrid, 2010-05-18) 6-well plate format (200K/well = 100K/ml)(1) No Dox control of both cells grew similarly before Day4, doubling time約為24h(2) #7 continued to grow between Day 4 and Day 7(3) #19 stopped to increase after Day 4 (猜測是第三天換 medium時同時移掉 死細胞)(4) YLC 將Day 8細胞拿來做SA-b-Gal staining,是positive, 但比例不詳 (AC 263 小夏) 6-well plate format (200K/well = 100K/ml)

上週末Bioruptor業務幫我們把儀器fix好的同時，也提及許多Lab在置備protein extract的同時，現在都會加一步sonication的步驟。 set at “Low”, 5 cycles. 據說這樣才可以充分地把胞膜上面的蛋白質都萃取出來。有做膜蛋白的人要注意一下! 非膜蛋白的人也可以試試看多加這一步驟，protein是不是萃取得更完全。