Lab Meeting

1. U0126 (MEK1/2 inhibitor) can inhibit EBV Rta induced p21 expression and EBV reactivation in EREV8.2. caspase 3 is involved in the survival activity of DDR1 in OSCC (在OEC-M1 and TW2.6 中knockdown DDR1看到pro form的減少)。3. 用autophage marker LC3B看看autophage是否參予著DDR1調控生長的機制。

No SFN signal in TW01TetEREV#27 cells may be due to GFP antibody-blocking. (Sorry didn’t think of it, I’ll check the probability)

1. To compare expression levels of various host proteins in TW01-EREV#27 with its parental cell TW01TetER#16.2. Test p53 and CDK6 antibodies from ICON.

報告這篇PAPER1. LMP1 increases the release of EGFR into exosomes and that purified exosomes containing LMP1 and EGFR are taken up by epithelial, endothelial, and fibroblast cells, leading to the activation of ERK and PI3K/Akt pathways.2. The ability to detect LMP1 in the exosome pellet harvested from serum of mice carrying the C15 tumor supports a

1. EBV Rta induces growth arrest in the G1 phase of cell cycle in TW01TetER cell lines (the effect is #19＞#7).2. Counting the viable cell by trypan blue exclusion method show that there is no difference between TW01TetER #7 and #19 in the growth rate for the period of 4 days.3. Briefly introduce the NPC

1. The RNAs of TW02, TW03, TW04, TW05, TW06 are ready. The RNAs of HONE1 and TWO1 will be purified on next week.2. EBV Rta can induce G1 arrest in TW01TetERTA #7, #16 and #19 in my 1st trial (19≧16＞7).3. So far, to test the inhibition effect of U0126 in ERTA-induced EBV reactivation.