Cancer Cell Vol 14: 146-
(1) RAD001 is effective in PIN solution by day 14 after treatment ) (PTEN null or AKT tg mice). By day2, S6 ribosomal protein and eIF4G are effectively inhibited by RAD001, but the p27 is still induced and the PIN is still present. By day 14 of RAD001 treatment, however PIN phenotype is resolved and the p27 is reduced to the normal concentration, suggesting neither mTOR nor AKT is required for p27-induced senescence program in prostatic intraepithelial neoplasia (PIN). (2) Induction of p27 checkpoint may be initiated during the detachment and inhibition of cell-cell contact. (3) (Me thought) should EBV Rta possesses function in quickly switching on transcription program that are involved in cell polarity?