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Gene ontological classification9 of the differentially expressed genes was performed by using the DAVID Bioinformatics Database functional-annotation tools (http://david.abcc.ncifcrf.gov/)30. The microarray data have been deposited in the Gene Expression Omnibus maintained by the US National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov/geo/).
Accession numbers. Gene Expression Omnibus series GSE9167.
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(a) Venn diagram of genes affecting SG and/or PB assembly. (b) Graphic depiction of functional classification of genes revealed with the DAVID bioinformatics tool.
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Analysis of hits using gene ontology terms.
We searched three databases, the Biobase BioKnowledge Library (BKL, http://www.biobase-international.com/pages/), David Bioinformatics Database (http://david.abcc.ncifcrf.gov/summary.jsp), and GeneSifter (http://www.genesifter.net/web/) for functional annotation of hits using gene ontology, a controlled vocabulary of gene descriptors. Annotations were derived from these databases in June 2007 and restricted to those supported by experimental evidence.
Note: Supplementary Information is available on the Nature Cell Biology website.