To test the effect of U0126 on EBV Rta-induced EBV reactivation on TW01EREV-2716
1. 初步結果72hr 20uM U0126死了一半的細胞 (WST-1 assay)
2. 感覺Dox 48小時活細胞有隨著U0126濃度的增加而增加
3. Dox 72小時細胞全部懸浮於medium，感覺72小時Dox作用效果遠大於U0126。(這樣可以說U0126是擋不住ER的Function? 也許可以像學姊早上說的降低Dox的量)
Next work will
1. Determine the cytotoxicity of U0126 on TW01EREV-2716 by WST-1 assay.
2. Confirm the expression kinetics (0-72hrs) of cell cycle regulators and viral proteins in Dox-treated TW01EREV-2716. (ex. SFN,p53)
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想確認一下:(1)是TWO1還是TW01?(2)是EREV#2716還是EREV2716?
還有,我覺得U0126的cytotoxicity先做出來,再來調整其它parameters.
(1)拍謝，是TW01。(2)改成TW01EREV-2716。