Janelle的部份

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由 sufang 在 六, 06/28/2014 – 16:03 發表 Oral Cancer DOK NIC nicotine Oral Cancer

Nicotine (NIC) – treated DOK: 針對NIC4, NIC5, NIC6 vs DOK1的array data, 配合日前所提, NIC-treated DOK clones 有明顯增加cell proliferation及migration的現象, 可能參與的pathway/genes有:
  1. DNA replication: MCM10 (3.37x), RECQL4 (2.32x), GINS2 (2.32x), CDC25A (3.01x), CDC7 (2.227x), NASP (2.19x), DTL (2.38x)。
  2. Cell cycle: E2F2 (2.22x), CDC6 (2.75x), CDC7(2.27x), CDC25A (3.01x), PKMYT1 (2.31x), CDKN1A (=p21, 0.44x), SOX9 (0.31x), HBP1 (0.38x), STEAP3 (0.4x), RPS6KA2 (0.22x), RPS6KA3 (0.39x)
  3. Transcriptional misregulation in cancers: SIN3A (0.52x), NUPR1 (0.39x)
  4. Fibroblast growth factor binding: TGFBR3 (3.63x), FGFBP1 (2.51x), FGFR3 (2.83x, NIC獨有的)
  5. TGF-beta signaling pathway: BMP4 (4.56x), BMP7 (8,98x)
  6. Focal adhesion: COL5A1 (0.49x), LAMA3 (0.38x), THBS3 (0.53x), ITGA3 (0.5x) , ITGA5 (0.37x), MET (0.49x), ITGB5 (0.49x), VEGFA (0.37x), VEGFC (0.5x), SHC1 (0.52x), MAP2K1 (0.52x), CTNNB1 (0.42x) 細胞focal adhesion能力下降?
  7. Cell adhesion: CLDN1 (0.46x), LPXN (0.34x), AMTN (0.21x) 細胞貼附能力下降?
  8. Stress fiber: GAS2L1 (2.65x), ENAH (2.26x), NEBL (2.67x)
  9. Epidermis development: KRT13 (75.39x, 可是不知為何去到40TN及4NQO mouse呈下降或不變趨勢)
小記一下:
–許多基因的上或下，AC和NNK較一致，但如果nicotine是由NNK來的，會期望看到nicotine與NNK較一致。先按下不表。
–KRT13被記載在下列這些GO中: tongue morphogenesis, keratin filament, intermediate filament, extracellular vesicular exosome, cellular response to retinoic acid, epidermis development, response to radiation

Arecoline (AC) – treated DOK: 這裡有兩組實驗結果可以看: 夏博的 DOK3 vs AC50-5 day (AC_short)以及靜娟的DOK2 vs AC50/AC100。先看兩組都有顯著變化的pathway/genes (括弧內為上升或下降倍數、先呈現AC50_short followed by AC50/AC100)：
  1. DNA replication: POLA1 (2.51, 2.94), POLA2 (2.01, 5.07), PRIM1 (2.08, 2.97), POLD1 (2.12, 2.38), POLE (2.00, 2.19), POLE2 (2.53, 5.66), MCM3 (2.01, 4.40), MCM5 (3.13, 7.34), MCM6 (2.37, 10.57), MCM7 (2.37, 5.0), RFC2 (2.14, 5.83), RFC3 (2.54, 8.99), RFC5 (2.00, 3.09), DNA2 (2.82, 3.45), RNASEH2A (2.15, 3.88), LIG1 (2.59, 3.00).
  2. Chromosome, centromeric region: BIRC5 (2.07, 8.35), CDCA8 (2.71, 8.17), CENPE (2.3, 8.25), CENPO (2.09, 5.48), CENPP (4.2, 3.45), CENPP (4.2, 3.45), DSCC1 (2.44, 6.68), HELLS (2.44, 5.45), HJURP (2.4, 8.14), INCENP (2.49, 3.68), MKI67 (2.39, 7 這個叫做Ki67!!), NUF2 (2.29, 6.31), SGOL1 (2.08, 7.69), SGOL2 (2.26, 4.35), SUV39H1 (2.04, 4.1), WDHD1 (2.04, 4.32).
  3.  Condensed chromosome kinetochore: BIRC5 (2.07, 8.35), BUB1B (2.48, 10.87), CASC5 (2.9, 3.78), CENPK (2.07, 9.71), CENPM (2.52, 4.8), CENPM (2.52, 4.8), CENPO (2.09, 5.48), ERCC6L (2.62, 7.39), HJURP (2.4, 8.14), INCENP (2.49, 3.68), MAD2L1 (2.14, 6.57), MLF1IP (2.97, 6.95), NUF2 (2.29, 6.31), SGOL1 (2.08, 7.69), SGOL2 (2.26, 4.35), SPAG5 (2.51, 3.98), SPC24 (3.28, 4.8), SPC25 (2.52, 10.83), ZWINT (3, 12.96).
 
NNK- treated DOK:

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