2014-01-20 冷泉港 CRISPR 說明會

大家好，

謹訂 1/20 下午1：30 在 R1-1041 舉辦 CRISPR 說明會，

由冷泉港的技術專員 陳博士與李小姐 負責解說，

內容包括 CRISPR 原理與應用、冷泉港 CRISPR 產品介紹與操作方式，

還有示範如何設計 guide RNA

如有其他問題或需求，歡迎現場提問，

請大家務必準時到場，謝謝

另外，當日的 Journal Club 順延一次

Maddalo D1, Manchado E1, Concepcion CP2, Bonetti C1, Vidigal JA1, Han YC1, Ogrodowski P1, Crippa A3, Rekhtman N4, de Stanchina E5, Lowe SW6, Ventura A1.

Author information

Abstract

Chromosomal rearrangements have a central role in the pathogenesis of human cancers and often result in the expression of therapeutically actionable gene fusions. A recently discovered example is a fusion between the genes echinoderm microtubule-associated protein like 4 (EML4) and anaplastic lymphoma kinase (ALK), generated by an inversion on the short arm of chromosome 2: inv(2)(p21p23). The EML4-ALK oncogene is detected in a subset of human non-small cell lung cancers (NSCLC) and is clinically relevant because it confers sensitivity to ALK inhibitors. Despite their importance, modelling such genetic events in mice has proven challenging and requires complex manipulation of the germ line. Here we describe an efficient method to induce specific chromosomal rearrangements in vivo using viral-mediated delivery of the CRISPR/Cas9 system to somatic cells of adult animals. We apply it to generate a mouse model of Eml4-Alk-driven lung cancer. The resulting tumours invariably harbour the Eml4-Alk inversion, express the Eml4-Alk fusion gene, display histopathological and molecular features typical of ALK(+) human NSCLCs, and respond to treatment with ALK inhibitors. The general strategy described here substantially expands our ability to model human cancers in mice and potentially in other organisms.