03/02 Lab Meeting-小嬿

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1. check Q-PCR primers: p21 and SFN–>Workable!2. ChIP test : a. antibodies: mouse IgG, CTCF and Flag antibody b. primers: H19ICR, SFN, GAPDH(coding region) c. results: (1) the problem of sonication is not dissolved (2) condition of Q-PCR is not well, but standard end-point PCR is OK (3) input is not equal. (one of them

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2/23 Lab Meeting-ingrid

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報告進度(1) TW05TetER：#1-18中，除了#1及#12以外，其餘在IFA中，為FLAG Ab陽性，重複做IFA(點片)以重複確認。(2) 以SK-N-SH cell lysates做OGT Ab的western analysis，覺得沒有認到目標蛋白質，再以Hela cell lysates當positive control及黃老師家的goat二抗再確認。

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AC384 Thaw BrCA cell lines for NTUH

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AC3842/17/2011Thaw HCC-70, HCC-2218, MDA-MB 134-VI, 157-VII, -361, -453。2/18/2011 change medium2//23/2011 cell count and sub-culture HCC-70: Total: 4 x 10∧5/ml*10-ml = 4x 10∧6 (1.3 M for T25, 2.7 M for p-10) HCC-2218: Total: 1.35 x 10∧5/ml*25-ml = 3.3x 10∧6 (1.1 M for T25, 2.2 M for p-10) MDA-MB134-VI: Total: 4.8x 10∧5/ml*9.5-ml = 4.8x 10∧6 (1.6 M

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線上抽籤 心誠則靈

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請閉目冥想三分鐘，潛心默誦心經 或觀世音菩薩聖號。 然後讓心靈自由浮現 1-100內任一數字，再點取籤詩內容。 01 11 21 31 41 51 61 71 81 91 02 12 22 32 42 52 62 72 82 92 03 13 23 33 43 53 63 73 83 93 04 14 24 34 44 54 64 74 84 94 05 15 25 35 45 55 65 75 85 95 06

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各種bytes間之換算

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kilobyte, megabytes, gigabyte, terabytes, petabytes, exabytes. Link is here. Processor or Virtual Storage Disk Storage · 1 Bit = Binary Digit· 8 Bits = 1 Byte· 1024 Bytes = 1 Kilobyte· 1024 Kilobytes = 1 Megabyte· 1024 Megabytes = 1 Gigabyte· 1024 Gigabytes = 1 Terabyte· 1024 Terabytes = 1 Petabyte· 1024 Petabytes = 1

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