Next generation sequencing in cancer research and clinical application

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http://www.biologicalproceduresonline.com/content/15/1/4 Recent NGS-based studies in cancer Cancer Experiment Design Description ref Colon cancer 72 WES, 68 RNA-seq, 2 WGS Identify multiple gene fusions such as RSPO2 and RSPO3 from RNA-seq that may function in tumorigenesis [15] Breast cancer 65 WGS/WES, 80 RNA-seq 36% of the mutations found in the study were expressed. Identify the abundance […]

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03/12- Progress Report-小嬿

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Fig. 1. CTCF repressed the lytic cycle replications of EBV and KSHV in viral latently infected cells.  > (a). The band of CTCF ectopic-expressed cells is over-exposed.  > (b). The expression level of shCTCF is similar to shLuc. Fig. 2. Rta binding sites are similar to CTCF binding sites Fig. 3. 4. EBV Rta expression

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Biomax tissue microarray informations

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Dear老闆: 這是tissue microarray的網址 http://www.biomax.us/tissue-arrays/Head_and_Neck/ 我挑了一下 (case多及有附normal control的) Bladder: BL2081 (203 cases) US. 455 BL802 (80 cases) US. 255 (兩片有60個cases重複) 建議買BL2081 Head and neck, oral cavity: Old: OR2081 (98 cases) US.455 (仍有trial等級 US.150) BC34011 (24 cases) US.140, HN811 (27 cases) US.215 New: HN483 (48 cases) US.165  (與OR2081有1個cases重複) HN803a (80 cases) US.255  (與OR2081有4個cases重複) 建議OR2081 trial等級+ HN803a 給你參考              

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Rta/CTCF MS related

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#2由 sufang 在 六, 05/09/2015 – 20:02 發表。Reviewers CommentsReviewers Comments From PLoS Pathogens (06/20–0/7/31, 6 wks) Reviewer #1: This is an interesting manuscript in which the model is advanced that EBV and KSHV Rta by binding to their respective genomes, induce local methylation which promulgates lytic replication and dissociation of CTCF, which is inhibitory to

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DDR1 manuscript 相關

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2015/05/08 (Thu)  MS submitted to Research Topic “Oral Oncology” 2015/05/30 (Sat)   MS returned, interactive reviews activated: Editor: Rui Amaral Mendes Catholic University of Portugal; Editor of J Carcinogenesis and Mutagenesis; Adhuant Prof at CWRU 2015/08/28 rejected… by Specialty Chief Editor Jean­Pascal Machiels     Reviewer#1: 加上morphology圖於Fig 1B band quantitation spheroid invasion   Reviewer#2: 移入IHC圖於Fig 5E 補上shRNA sequences and offtarget

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FGFR fusion related

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2/27(四)合作討論會議: BGJ398 A2204 cholangiocarcinoma study #1由 sufang 在 三, 04/09/2014 – 12:08 發表。 Norvatis人員的報告摘要 1. K650E in FGFR3 mutation is also sensitive to BGJ398. 2. Dan/YiMi的paper是K652E對PD173074無效。 3. TKI258不知道是啥…有點失憶!

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20140212 Lab meeting – ingrid

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LabMeeting-Ingrid由 EVKVLIN 在 三, 02/12/2014 – 00:00 發表 LIN Lab 2016 Ingrid LabMeeting YCK LabMeeting-Ingrid 由 EVKVLIN 在 三, 02/12/2014 – 00:00 發表  LIN Lab 2016   Ingrid   LabMeeting   YCK 2008/09/09 Lab Meeting 心得彙整 Ingrid: (1) Preparation of GST-KR for polyclonal antibodies: protocol okay, 但把benzonase加入resuspending buffer中,而protease inhibitor放在第一次thaw完的lysate中,如果還是很黏的話要在thaw完後sonicate 15~30秒 (2)  366/367EE 有抑制現象!請再repeat一次,並且加入pFA-系列,如果也是抑制：(A) 以IP確認WT,366AA及366EE和HDAC1之interaction(we’ve bought this ab, didn’t

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