2014-02-18 Journal Club Paper
Title:Claudin-2 Promotes Breast Cancer Liver Metastasis by Facilitating Tumor Cell Interactions with Hepatocytes by En-Wei(原文下載)
2014-02-18 Journal Club Paper Read More »
Title:Claudin-2 Promotes Breast Cancer Liver Metastasis by Facilitating Tumor Cell Interactions with Hepatocytes by En-Wei(原文下載)
2014-02-18 Journal Club Paper Read More »
LabMeeting-Ingrid由 EVKVLIN 在 三, 02/12/2014 – 00:00 發表 LIN Lab 2016 Ingrid LabMeeting YCK LabMeeting-Ingrid 由 EVKVLIN 在 三, 02/12/2014 – 00:00 發表 LIN Lab 2016 Ingrid LabMeeting YCK 2008/09/09 Lab Meeting 心得彙整 Ingrid: (1) Preparation of GST-KR for polyclonal antibodies: protocol okay, 但把benzonase加入resuspending buffer中,而protease inhibitor放在第一次thaw完的lysate中,如果還是很黏的話要在thaw完後sonicate 15~30秒 (2) 366/367EE 有抑制現象!請再repeat一次,並且加入pFA-系列,如果也是抑制:(A) 以IP確認WT,366AA及366EE和HDAC1之interaction(we’ve bought this ab, didn’t
20140212 Lab meeting – ingrid Read More »
From pdf 2793: KLF4, p21 and context-dependent opposing forces in cancer Benjamin D. Rowland and Daniel S. Peeper
2006 Pdf 2793. Good Figures Read More »
地點: 台南統一大樓1/25/2014 (Saturday) 和信醫院鄭鴻鈞醫師:-OrCA: 1405 (Frozen tissues)-NPC: 1206 (Frozen tissues)-Biobank and Informed Consent Form (ICF) 有研究要進行才會去簽ICF-在該醫院開刀的,約有一半的檢體留存率。-800多例檢體要做expression array. 基因表達平台。 劉柯老師: -乙醛Acetaldehyde, ROS, Nitrosamine-P. gingivalis/C.albicans (TLR2 -> IL10 ->Treg)-P.gingivalis LPS -> miR-146a increases-P.gingivalis LPS ->TLR2-JNK, low inflammation-E. coli LPS -> TLR4 /p38MAPK, TLR4-NFKB-Areca nut -> increases miR-146a Jeffery:-Infection: 1. Direct: HPV 2. Indirect: inflammation (P. gingivalis), carcinogens,
2014/01/25-26 PI Retreat Read More »
整理檔案時撈回來的圖 Nature Genetics 38, 489 – 494 (2006) Published online: 19 March 2006; | doi:10.1038/ng1755
Functional classification of drugs by properties of their pairwise interactions Read More »
TCGA data download 上次回去尾牙 Dr. Lin 提到TCGA的DATA DOWNLOAD 目前找到的地方是這裡 https://tcga-data.nci.nih.gov/tcga/dataAccessMatrix.htm 我有試著下載 HNSC的miRNAseq的data跟clinical data 看data matrix 其中miRseq的data 應該是已經處理過的 給的數值是count 跟 frequency 但是會有不同read frame的問題 我想 gene expression 應該也會有類似問題 目前還是不知道怎麼處理 跟NGS資料分析 一點都不熟阿! XDDDDD
1. 整理與Harvard Skin Disease Research Center Dr.Rheinwald購買之細胞株培養方法 2. 試著釐清Src與DDR1的活性之關聯性: 過去研究指出(1)Src innibitor會抑制collagen I induced DDR1的活性(2)Src會與p-DDR1有交互作用 我的結果指出(1)imatinib處理會抑制Src的活性 (2)比較不同株細胞Src的活性與DDR1的活性似乎有相關 (3)加入Src innibitor會影響OEC-M1中DDR1與Src的交互作用(此現象在TW2.6不明顯) (4)加入Src innibitor 對於OSCC細胞生長的抑制影響不是很明顯(1uM濃度, 48小時) 3. 整理過去發現Collagens處理後DDR1,ITG..等mRNA level增加的例子, 結論為大多條件均在serum free下進行, 因此會嘗試改變為這樣條件去觀察在keratinocytes中處理Collagens對DDR1及ITGs mRNA level的影響 4. QPCR的CT值差異約要載1.5-2以上 以終產物跑agarose gel 才看的出來差異
20140115 Lab meeting-小丸子 Read More »
Dear all: 隨信附上下週(1/21)journal分享的paper,題目為 Grhl3/Get1 suppresses miR-21 expression and tumorigenesis 請查收 The Grainyhead transcription factor Grhl3/Get1 suppresses miR-21 expression and tumorigenesis in skin: modulation of the miR-21 target MSH2 by RNA-binding protein DND.
20140121 Journal Club paper Read More »