2014

LabMeeting-小丸

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LabMeeting-小丸由 EVKVLIN 在 二, 03/25/2014 – 00:00 發表 LIN Lab 2016 LabMeeting maruko 小丸2008/09/09 Lab Meeting 心得彙整 Maruko:要不要考慮這個跟這個,還有一個這個. 另外S186A是一個至今仍然未解的有趣mutant. 它可以bind DNA,可以transactivate一些promoter,有被訂出in vivo是被PKC磷酸化,但似乎此位點磷酸化的有無與disrupt latency無關.所以此例子並不適合追太久.. 2008/09/16 Lab Meeting 心得彙整 Maruko: (1) 為確保293TetKR中仍是每個細胞均具有transgene,小丸子成功地以flow demonstrated that, although weak, but Dox treated 293TetKR homogeneously shifted the anti-flag staining (1:50) to the right (not seen in 1:100 staining). 這樣子的結果可以讓我們安心的繼續使用這個細胞株.當初其實很怕是部份細還有KR,部份細胞則沒了,而以immunoblotting 又分不出來,所以小丸子勇猛地做這個實驗(熱烈鼓掌 霹靂啪啦 霹靂啪啦)智者常言:打鐵趁熱,所以讓我們也篩一下ER.能否請小蓮協助/學習小丸子把手中的293TetLuc及TetER也做一次這樣的實驗,我猜KR不幸是最弱的表現者. […]

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Next generation sequencing in cancer research and clinical application

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http://www.biologicalproceduresonline.com/content/15/1/4 Recent NGS-based studies in cancer Cancer Experiment Design Description ref Colon cancer 72 WES, 68 RNA-seq, 2 WGS Identify multiple gene fusions such as RSPO2 and RSPO3 from RNA-seq that may function in tumorigenesis [15] Breast cancer 65 WGS/WES, 80 RNA-seq 36% of the mutations found in the study were expressed. Identify the abundance

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03/12- Progress Report-小嬿

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Fig. 1. CTCF repressed the lytic cycle replications of EBV and KSHV in viral latently infected cells.  > (a). The band of CTCF ectopic-expressed cells is over-exposed.  > (b). The expression level of shCTCF is similar to shLuc. Fig. 2. Rta binding sites are similar to CTCF binding sites Fig. 3. 4. EBV Rta expression

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Biomax tissue microarray informations

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Dear老闆: 這是tissue microarray的網址 http://www.biomax.us/tissue-arrays/Head_and_Neck/ 我挑了一下 (case多及有附normal control的) Bladder: BL2081 (203 cases) US. 455 BL802 (80 cases) US. 255 (兩片有60個cases重複) 建議買BL2081 Head and neck, oral cavity: Old: OR2081 (98 cases) US.455 (仍有trial等級 US.150) BC34011 (24 cases) US.140, HN811 (27 cases) US.215 New: HN483 (48 cases) US.165  (與OR2081有1個cases重複) HN803a (80 cases) US.255  (與OR2081有4個cases重複) 建議OR2081 trial等級+ HN803a 給你參考              

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Rta/CTCF MS related

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#2由 sufang 在 六, 05/09/2015 – 20:02 發表。Reviewers CommentsReviewers Comments From PLoS Pathogens (06/20–0/7/31, 6 wks) Reviewer #1: This is an interesting manuscript in which the model is advanced that EBV and KSHV Rta by binding to their respective genomes, induce local methylation which promulgates lytic replication and dissociation of CTCF, which is inhibitory to

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DDR1 manuscript 相關

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2015/05/08 (Thu)  MS submitted to Research Topic “Oral Oncology” 2015/05/30 (Sat)   MS returned, interactive reviews activated: Editor: Rui Amaral Mendes Catholic University of Portugal; Editor of J Carcinogenesis and Mutagenesis; Adhuant Prof at CWRU 2015/08/28 rejected… by Specialty Chief Editor Jean­Pascal Machiels     Reviewer#1: 加上morphology圖於Fig 1B band quantitation spheroid invasion   Reviewer#2: 移入IHC圖於Fig 5E 補上shRNA sequences and offtarget

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FGFR fusion related

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2/27(四)合作討論會議: BGJ398 A2204 cholangiocarcinoma study #1由 sufang 在 三, 04/09/2014 – 12:08 發表。 Norvatis人員的報告摘要 1. K650E in FGFR3 mutation is also sensitive to BGJ398. 2. Dan/YiMi的paper是K652E對PD173074無效。 3. TKI258不知道是啥…有點失憶!

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