20140212 Lab meeting – ingrid

LabMeeting-Ingrid由 EVKVLIN 在 三, 02/12/2014 – 00:00 發表 LIN Lab 2016 Ingrid LabMeeting YCK LabMeeting-Ingrid 由 EVKVLIN 在 三, 02/12/2014 – 00:00 發表  LIN Lab 2016   Ingrid   LabMeeting   YCK 2008/09/09 Lab Meeting 心得彙整 Ingrid: (1) Preparation of GST-KR for polyclonal antibodies: protocol okay, 但把benzonase加入resuspending buffer中,而protease inhibitor放在第一次thaw完的lysate中,如果還是很黏的話要在thaw完後sonicate 15~30秒 (2)  366/367EE 有抑制現象!請再repeat一次,並且加入pFA-系列,如果也是抑制:(A) 以IP確認WT,366AA及366EE和HDAC1之interaction(we’ve bought this ab, didn’t […]

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