April 2012

Rta/p21/Senescence/Tumorigenesis

IB Roninson pdf 1605, 1624 in Bound Book (Senescence 3/3) PDF 1624: Short-term expression of p21 led to rapid downregulation of genes involved in mitosis and DNA replication. However, a longer-term consequence of p21 induction resulted in the induction of multigenes (PDF 1605), many of which are involved in aging as well as secreting anti-apoptotic […]

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0409 Lab meeting-蔡小丸

1. 將DDR1 IP後的interacting proteins (no. 1-4) 切下後訂mass, 而no. 1約60 kDa, no. 2約75kDa, no. 3約85 kDa, no. 4約160 kDa的蛋白質, 其中最有興趣在一些OSCC細胞中可能有高度磷酸化的區域為no.2 利用MASCOT分析的結果統 整出較有可能的protein分別為 Heat shock 70 kDa protein 8, Heat shock 70 kDa protein 9 及 Polyadenylate-binding protein 1, 這些結果進一步需實驗進行驗證2. 利用OEC-M1處理Glivec後, 觀察抑制DDR1活性對OEC-M1的影響 結果發現細胞生長停止, 但細胞死亡的情形不明顯3. 觀察K2及K6細胞對Glivec的敏感性, 結果發現Glivec對於DDR1表達量較少的K2的生長抑制效果較差, 此結果仍須重複確認

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Let’s Move On!

4/10 SFL wrote:Sorry FYC, 多兩篇給你! (ref new 1), (ref new 2)。另外, 下面的ref 3–7你可以先忽略不必看。—————————- 恭喜小丸子、Ingrid得獎!!也謝謝小夏、小嬿的努力,我們和knicks一樣→work as a team,個人獲獎就是整個team的榮耀! 所以請客這種事就是老闆出錢、夥計們出力就對了! 往下大家(包括FYC)的目標調整如下: 1. 要present的圖,包括平日lab meeting均要run一下統計。 2. EBV related: (a) Infection of NP460hTert with Akata-GFP EBV  (ref 1, ref 2) (b) Rta-mediated genome organization (ref 3, ref 4, ref 5, ref 6, ref 7)。3. Oral cancer related : (a)

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