Studying plans: 1. How is DDR1 regulated by miRNA? — We will discuss the detail. 2. How many signaling pathways are involved in oral tumorigenesis? How many signaling pathways are involved in oral premalignant lesions? — There are 12 important signaling pathways invovled in tumorigenesis. 3. Is EGCG working on 12 signaling pathways? Is […]
Summary for the discussion Read More »
cd Desktopvi “program name”#!/usr/bin/perl (Hash=shift3 Bang=shift1)print “hello worldn”
1. 完成三組tissue microarray的DDR1, collagen type 1及collagen type 4的染色, 初步計分結果, 似乎DDR1的大量表達與collagen type1的大量表達較為相關,而DDR1大量表達也與疾病的grade較相關2. 比較OSCC cells DDR1 tyrosine phosphorylation的情形, 結果為DOK, C9, OC-3, OEC-M1, TW2.6>HSC-3, SCC-15, T47D>HEK293, 發現DDR1 tyrosine phosphorylation的程度與gilvec對於這些細胞的抑制能力,沒有很好的相關性, 然而發現在OEC-M1, TW2.6, HSC-3, SCC-15這幾組IP DDR1 sample中, 發現約略80-85kDa的DDR1-associated protein有強烈的tyrosine phosphorylation, 但此蛋白為何未知3.此外 DDR1的活性反映出的下游為何未知 (但排除非p-MAPK及p-AKT)4.利用Q-PCR分析一系列oral cells的DDR1 isoforms的表達, 結果發現大多細胞只表達1a及1b這兩種isoforms, 而mRNA表達量也與蛋白表達量具相關性
0215Lab meeting-蔡小丸 Read More »
1. 下次開會: 6月2. CA OrCA PIs + 王副院長、莊JL、陳YR、黃HG (RA:雪君)、江主秘。3. BioSignature Program: Leader/Chang Gung 核醫科主任閻紫宸, looking for cause-effect marker. The third meeting will be held on March-8; focus on miRNA/epigenetics/DNAs; funding source, Academia Sinica.4. 王副院長Lab學生報告 (Wei-Chieh Huang)—長庚閻醫師 has 53 OrCA T/N pairs; miR-491-5p is involved in cell migration and invasiveness, targeting GIT1 and RAB1; OC-3 and
2/13/2012 OrCA Meeting Read More »
1. 計數TW05TetLuc/ER dox treat 1, 2, 3, 5, 6, 8天的細胞數。2. 以467確認Rta expression pattern 在TW01TetER及TW05TetER不同。 Rta expression level by WB: #19 ≧#16＞TW05TetER。 將再確認p21, c-Myc, p53……的表現。3. 準備HEK293, DOK, HSC3, SCC15, OECM1的genomic DNA來確認DDR1的mutation位點。4.準備HEK293, DOK, HSC3, SCC15, OECM1的cDNA來確認DDR1的isoform。
2012/2/1 Lab Meeting- 小夏 Read More »