03/02 Lab Meeting-小嬿
1. check Q-PCR primers: p21 and SFN–>Workable!2. ChIP test : a. antibodies: mouse IgG, CTCF and Flag antibody b. primers: H19ICR, SFN, GAPDH(coding region) c. results: (1) the problem of sonication is not dissolved (2) condition of Q-PCR is not well, but standard end-point PCR is OK (3) input is not equal. (one of them […]
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