January 27, 2011

1/26 Lab Meeting-ingrid

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報告進度 (1) TW03Tet：因挑選細胞株TetR表現差，故重新挑選。TW05Tet：以#4進行接下來的穩定細胞株篩選 (#3、#4及#6送入液態氮)，包括Flag-Luc、Flag-gZ+Rta(1：3)、Flag-Rta (2)以shLuc、shOGT、shMGEA5的lentivirus (稀釋倍數1/8) 送入ERKV (2.4×10^5/ml/well/12well) 中，24、48、72小時後，收lysates進行WB確認目標蛋白質是否有減少。 (3)以SK-N-SH cell lysates當作OGT Ab的positive control，同時確認goat二抗是否可用。

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01/26 Meeting 小嬿

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Progress Report: 1. The stability of EBV Rta a. the expression level of RTS-15 and Flag-Rta is similar but slightly higher than Rta-V5. b. Rta expression level of 6-hr transfection is blocked by MG115 and fragmented into smaller size, and the fragments are stablized by MG115 treatment.2. The expression level of Akata-EGFP/EBV and Akata-p2089 is

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