四神湯成份
淮山、芡實、茯苓、蓮子。
2010
11/10 Lab Meeting- ingrid
1. 想要測試Dox對TW01Tet的生長是否有所影響，結果失敗。下次要收短時間測試(如1、2、3天)。 2. 篩選TW03Tet及TW05Tet細胞株。TW03Tet：24株裡有19株長起來；TW05Tet：24株裡有12株長起來。目前皆放在6 well內等待Western blot測試TetR。 3. OGT抗體無法辨認抗原，已重新購買。另外要解決internal control在每個sample中的量皆不相同的問題。 4. 將shMGEA5送入ERKV細胞中，”似乎”可以使發紅光的細胞比例上升，要再進一步詳細確認。
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33 Lines
33 Lines由 sufang 在 日, 11/07/2010 – 00:00 發表 Pre-published 33 Lines Cell Lines Dan Robinson PAPA PTK profiling RAGE Cell Designation Tissue 1 Tissue 2 Morphology SW-13 Adrenal gland cortex, primary small cell carcinoma Epithelial T24 Bladder transitional cell carcinoma Epithelial SW-1353 Bone chondrosarcoma Fibroblast Saos-2 Bone Osteosarcoma Epithelial
11/3 Lab Meeting- 小夏
1. EBV Rta induces growth arrest in the G1 phase of cell cycle in TW01TetER cell lines (the effect is #19＞#7).2. Counting the viable cell by trypan blue exclusion method show that there is no difference between TW01TetER #7 and #19 in the growth rate for the period of 4 days.3. Briefly introduce the NPC
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10/27 Lab meeting 蔡小丸
整理departmental seminar時 大家的問題與建議接下來要進行的部分:1. DDR1是否是constutively active form2. 延長shDDR1給予時間 觀察細胞反應3. 不同稀釋之病毒液 是否能有dose的抑制效果 或要選擇不同的shDDR1 clone4. 加入其他oral cancer cells
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10/27 Lab Meeting- ingrid
測試從中研院買來針對OGT及MGEA5的shRNA系統1. 簡單以質體轉染送入細胞，再做Q-PCR，發現二者都可以成功抑制RNA的表現2. 包lentivirus感染送入細胞，再做Western blot analysis，發現可以成功抑制MGEA5蛋白質的表現。但OGT抗體尚未抓到條件，所以還未得知是否也有抑制。
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10/27 Lab Meeting- 小夏
1. The RNAs of TW02, TW03, TW04, TW05, TW06 are ready. The RNAs of HONE1 and TWO1 will be purified on next week.2. EBV Rta can induce G1 arrest in TW01TetERTA #7, #16 and #19 in my 1st trial (19≧16＞7).3. So far, to test the inhibition effect of U0126 in ERTA-induced EBV reactivation.
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10/27 Lab Meeting- 小嬿
1. Establishment of several stable cell lines: Akata-p2089, TW01EREV-Akata/EGFP, TW03Tet, and TW05Tet. I need YCK’s help to screen TW03Tet and TW05 Tet cells. I’ll transfer these clones to YCK tomorrow. 2. To test sonication condition of ChIP.
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10/21 Lab meeting-蔡小丸
繼續報告DDR1 對於台灣的oral cancer cell lines的重要性並再討論 DDR1 mediated cell growth可能會經由 MAPK surivial pathway及嚼檳榔和 OSCC大量表達DDR1的關聯性 再度謝謝大家連三周 被我疲勞轟炸 及給我的寶貴建議
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