11/24 Lab Meeting-蔡小丸
1. 除了OEC-M1以外 其他的口腔癌細胞株都沒有mycoplasma的污染2. 多株口腔癌細胞株DDR1表達量之比較3. shDDR1及Glivec對於非台灣地區 口腔癌細胞的影響4. 持續測試OSCCs之DDR1 activity (phospho-Tyrosine status)
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November 2010
11/17 Lab Meeting 小嬿
1. Establishment of Akata-p2089, TW01EREV-Akata-EGFP cell lines. 2. To test the sonication condition of ChIP. 3. To evaluate the expression level of X protein in 293- and TW01-series cell lines.
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11/17 Lab Meeting- 小夏
報告這篇PAPER1. LMP1 increases the release of EGFR into exosomes and that purified exosomes containing LMP1 and EGFR are taken up by epithelial, endothelial, and fibroblast cells, leading to the activation of ERK and PI3K/Akt pathways.2. The ability to detect LMP1 in the exosome pellet harvested from serum of mice carrying the C15 tumor supports a
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11/10 Lab Meeting- ingrid
1. 想要測試Dox對TW01Tet的生長是否有所影響，結果失敗。下次要收短時間測試(如1、2、3天)。 2. 篩選TW03Tet及TW05Tet細胞株。TW03Tet：24株裡有19株長起來；TW05Tet：24株裡有12株長起來。目前皆放在6 well內等待Western blot測試TetR。 3. OGT抗體無法辨認抗原，已重新購買。另外要解決internal control在每個sample中的量皆不相同的問題。 4. 將shMGEA5送入ERKV細胞中，”似乎”可以使發紅光的細胞比例上升，要再進一步詳細確認。
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33 Lines
33 Lines由 sufang 在 日, 11/07/2010 – 00:00 發表 Pre-published 33 Lines Cell Lines Dan Robinson PAPA PTK profiling RAGE Cell Designation Tissue 1 Tissue 2 Morphology SW-13 Adrenal gland cortex, primary small cell carcinoma Epithelial T24 Bladder transitional cell carcinoma Epithelial SW-1353 Bone chondrosarcoma Fibroblast Saos-2 Bone Osteosarcoma Epithelial
11/3 Lab Meeting- 小夏
1. EBV Rta induces growth arrest in the G1 phase of cell cycle in TW01TetER cell lines (the effect is #19＞#7).2. Counting the viable cell by trypan blue exclusion method show that there is no difference between TW01TetER #7 and #19 in the growth rate for the period of 4 days.3. Briefly introduce the NPC
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10/27 Lab meeting 蔡小丸
整理departmental seminar時 大家的問題與建議接下來要進行的部分:1. DDR1是否是constutively active form2. 延長shDDR1給予時間 觀察細胞反應3. 不同稀釋之病毒液 是否能有dose的抑制效果 或要選擇不同的shDDR1 clone4. 加入其他oral cancer cells
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