2008 Nobel Prize in Chemistry
詳情見這裡, 照片見這裡, 中文版見這裡 ~ 得主是Osamu Shimomura (下村脩) (從水母中發現GFP) Martin Chalfie (應用GFP於C.elegan 之研究, 確立其bio-marker地位) Roger Tsien (錢永健) (突變GFP, 使其顏色變化)
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2008
10/07 Lab Meeting 心得彙整
Ingrid:DLR western blot analysis沒有測出Flag-bands; pFA-GAL4系列質體活性測試(1:5+EF1-RL為internal ctrl)實驗已完成,等OrionL發完脾氣後才可得到實驗結果;著手進行7個T366及T367(含WT)全長的expression與DLR assay. Maruko:Flag-IP有初步結果,繼續微調其它調件;latency disruption籌劃中;全長的pLenti系列DLR操作完畢,等待機器修復中;slide上那個”pAN-Luc”要改為PANp-Luc或PAN-Luc(這是學他們做ChIP-chip的人們說的)PAN stands for polyadenylated nuclear 是本人10年前花了好幾個晚上想出的名子,請務必保留…(另外一個UCSF group想出的叫nut=nuclear transcript=T1.1,我覺得我的小孩名子叫起來比較好聽耶.嘿嘿嘿,老灰阿說起歷史就停不下來,sorry)
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2008 Nobel medicine prize
詳情請見這裡, 照片見這裡… 得主是Harold zur Hausen (發現HPV造成cervical cancer) Francoise Barre-Sinoussi及 Luc Montagnier (自愛滋病患者組織分離出HIV) 嗯…HPV及HIV都得獎了, 我們EBV輸了~~~ 還有, zur Hausen是那位editor吧 ? (心情大好? 看來paper接受定了吧 …)
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RNA Pol II Pausing
Richard Young (2/2)Nature Genetics: 2007 Dec Figure 1 Different classes of Pol II binding profiles. ChIP-chip assays were carried out with 2-4 h Toll10b embryos using antibodies that recognize both the initiating and the elongating forms of Pol II. Figure 2 Whole-genome analysis of Pol II binding.
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封網通知
Dear諸作者:因要po一些較detailed/confidential的文,今天下午以後一個禮拜內,麻煩你們進來時登錄一下,或是讓account保持登入狀態,這樣就不用重複sign-in/log-out了.造成不便,請多包涵.
又到週末了
那麼,祈願美國經濟不可崩盤,歐盟諸公繼續挺台灣入衛,台灣八點檔連續劇恢復一個鐘頭,青菜水果價格恢復…（喂喂喂,大家多多開文啊,這裡是”Our Thoughts”~寫了不愛還可以自己消掉啊.也許大家都忙著做實驗吧,看來當老闆還是不錯的)下週二Lab meeting時,小嬿要報告RNA Pol II pausing的paper(rehearsal).現在請各位捫心自問:R/Z這些傢伙是怎樣幫忙RNA Pol II做病毒基因轉錄的?
TPA,PKC,G2/M arrest
1998 Metastatic melanoma1999 PKC/p21/G2/M 2001 TPA/p21/morphological change2006 Pancreatic cell TPA/G2M2006 HepG2/TPA and previous study in 2003
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歡迎新作者
拍拍手,我們歡迎你,旻潔!(確定不用藝名嗎?)(又,依據老鼠會原則可否請把Sheng-Yen的email給Reactivation(sflin3@gmail.com)我等他過一陣子較不忙時再邀他加入作者群). Many thanks, and again, welcome!
訊息佈達(更新版/最終話)
On Sep-16-2008 AM 10:00, SFL wrote:Dear All, 陳老師在上週三院務會議後,熱切地吩咐我要把Rta造成MN增加的現象(楚穎論文)再加幾個圖(仿常申之paper)希望Int. J. Cancer這一篇被接受後,能夠把Rta對GI的contribution由我們Taiwan人自己發表(餘略)—————————————————————————–三個成見(二不一會):1. Rta’s function in the cytoplasm is NOT considered here.2. Rta-mediated MN formation in cells during EBV lytic replication may NOT contribute to NPC tumorigenesis, because cells may die before MN are formed.3. Rta does lead to MN and rH2AX formations in EBV-ve cells. Rta, AKT/ERK